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KMID : 0379119810090010001
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Korean Journal of Mycology
1981 Volume.9 No. 1 p.1 ~ p.6
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Producibility of Aflatoxin by Aspergillus parasiticus in Barley and Their Rediosensitivity
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Pericles Markakis
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Abstract
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The effect of gamma irradiation on production and accumulation of affatoxin on natural substrate (barley) by Aspergillus parasiticus NRRL 2999 has been studied in some detail_ Gamma irradiation at five doses, 0. 50, 100, 200 and 400 Krad was applied to the grain either soon after moisture equilibration (3 days after inoculation) or 10 days later (13 days after inoculation). And the results were as in the followings.
1. Increase in moisture content from 17% to 25% greatly increased the aflatoxin concentration, especially at zero irradiation dose. 2. Prolongation of the incubation period prior to irradiation from 3 to 13 days resulted in greater accumulation of aflatoxin. 3. Two hundreds Krad applies 13 days after inoculation on barley stored at 25% moisture (100% RH) and 25℃ led to higher aflatoxin production than 100 Krad or even 50 Krad. 4. The relative proportion of the principal aflatoxins in relation to irradiation showed that aflatoxin G was elaborated at a significantly higher rate than aflatoxin B.
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